Project Executive Summary

The current project offers a unique opportunity to prepare for prospective attacks with biological agents, but is addressing in the same time to the organizations and scientists involved in operational surveillance of medically important vectors and pests. This is achieved by combining breakthrough technological and scientifical developments to produce a unique detection kit concept.

The main product of TicKitqPCR is meant to be an in vitro PCR nucleic acid amplification test for qualitative and quantitative detection of genomes of Borrelia burgdorferi sl., Francisella tularensis, tick-borne encephalites virus (TBEv), and Crimean–Congo haemorrhagic fever virus (CCHFv), in biological materials (ticks, blood, cerebrospinal fluid, and animal tissue), by using real-time hybridization-fluorescence detection.

A key innovation of TicKitqPCR is that these molecular biology methods and tools will have a wide variety of potential applications in targeting operational surveillance, disease control, and national security management and planning. The performed studies will strengthen the national and international research by providing a kit allowing researchers from various fields of research to analyse biological samples in a fast and convenient manner, thus increasing the screening throughput and enhancing the competitiveness of the participants.

Among the beneficiaries of this project’s results, national security and public health policies implementation and elaboration institutions are included.

TicKitqPCR will contribute significantly to the national societal objectives of security, and health maintenance, through the generation of a rapid pathogen detection tool.